Cas 3 immo off3/28/2024 ![]() Only one off-target indel mutation was identified from the WGS data and validated by Sanger sequencing. Subsequently, 3272 potential off-target sites were selected for further analysis. In total, we identified between 202,008 and 272,397 single nucleotide polymorphisms (SNPs) and between 26,391 and 55,414 insertions/deletions (indels) in the seven Cas9-edited grapevine plants compared with the three WT plants. To assess the off-target mutation rate in grapevine ( Vitis vinifera), we performed whole-genome sequencing (WGS) of seven Cas9-edited grapevine plants in which one of two genes was targeted by CRISPR/Cas9 and three wild-type (WT) plants. However, the specificity of Cas9 targeting is poorly investigated in many plant species, including fruit trees. ![]() The CRISPR (clustered regularly interspaced short palindromic repeats)-associated protein 9 (Cas9) system is a powerful tool for targeted genome editing, with applications that include plant biotechnology and functional genomics research.
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